Phosphate → Sodium Phosphate, Dibasic

HRID 2059499Notes

Reaction equation

HRID 2059499 reaction equation
[P:1]([O-:5])([O-:4])([O-:3])=[O:2].[OH:6][P:7]([O-:10])([OH:9])=[O:8].[Na+:11].OP([O-])([O-])=O.[Na+].[Na+]>O>[OH:3][P:1]([O-:5])([O-:4])=[O:2].[Na+:11].[Na+:11].[P:7]([O-:10])([O-:9])([O-:8])=[O:6].[Na+:11].[Na+:11].[Na+:11]

Participants

6

Reactant 3

HCID 1061PhosphateHCID 24203Sodium Phosphate, Dibasic2×HCID 23672064Monosodium phosphate3×

Product 2

HCID 24203Sodium Phosphate, DibasicHCID 24243Trisodium Phosphate

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

Stock solutions for phosphate buffers were prepared as follows: 0.5 M solution of sodium phosphate monobasic (FW=119.96) was made by dissolving 59.98 g sodium phosphate monobasic in a final volume of 1 L of water. 0.5 M solution of sodium phosphate dibasic (FW=141.96) was prepared by dissolving 70.98 g sodium phosphate dibasic in a final volume of 1 L of water. A stock of 0.5 M sodium phosphate buffer was prepared by mixing 0.5 M sodium phosphate monobasic and 0.5 M sodium phosphate dibasic while stiffing and monitoring until the desired pH was reached. The stock buffer was diluted as appropriate to reach the desired concentration and salt was added as necessary to reach the desired conductivity. Sodium phosphate buffer, pH 6.5 is a 50 mM sodium phosphate buffer, pH 6.5 containing 35 mM NaCl made by mixing 100 ml 0.5 M Sodium phosphate buffer, pH 6.5 and 7 ml 5 M NaCl in water to a final volume of 1 L. The buffer was filtered, degassed and the conductivity was measured before use. Conductivity of the buffer was recorded as ˜8 mS/cm. Sodium phosphate, pH 7.4 is a 50 mM sodium phosphate buffer, pH 7.4 containing 10 mM NaCl was made by mixing 100 ml sodium phosphate buffer, pH 7.4 and 2 ml 5 M NaCl in water to a final volume of 1 L. The buffer was filtered, degassed and the conductivity was measured before use. Conductivity of the buffer was recorded as: ˜7.2 mS/cm.

Workup

  1. additionThe stock buffer was diluted as appropriate
  2. concentrationconcentration and salt
  3. additionwas added as necessary
  4. additioncontaining 35 mM NaCl
  5. additionby mixing 100 ml 0.5 M Sodium phosphate buffer, pH 6.5 and 7 ml 5 M NaCl in water to a final volume of 1 L
  6. filtrationThe buffer was filtered
  7. customdegassed
  8. additioncontaining 10 mM NaCl
  9. additionby mixing 100 ml sodium phosphate buffer, pH 7.4 and 2 ml 5 M NaCl in water to a final volume of 1 L
  10. filtrationThe buffer was filtered
  11. customdegassed

Sources & evidence

ORD record
ord-a21637a1cdaf48c0b3b7e69cc912f0b2
Source dataset
uspto-grants-2012_10
Patent number
US08277649B2

Reaction notes

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