Protocatechuic Acid → L-Glutamic Acid

Participants

Reaction Steps

On the CM2B agar medium, the 2A-1R strain could form colonies at substantially the same rate as that of the wild-type strain ATCC13869. However, on the minimum plate medium (20 g/l glucose, 2.64 g/l ammonium sulfate, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 0.25 g/l MgSO4.7H2O, 0.01 g/l FeSO4.7H2O, 0.01 g/l MnSO4.7H2O, 0.01 g/l CaCl2, 0.02 mg/l CuSO4, 40 g/l MOPS, 30 mg/l protocatechuic acid, 200 μg/l VB1.Hu, 300 μg/l Biotin, 20 g/l agar, adjusted to pH 6.7 with NaOH), the 2A-1R strain showed a considerably decreased colony-forming rate as compared to the wild-type ATCC13869 strain. Accordingly, a gene that can recover the growth of the 2A-1R strain in the minimum medium was found.

Provenance & Evidence