Protocatechuic Acid → L-Glutamic Acid

HRID 2059547Notes

Reaction equation

HRID 2059547 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].[Cl-].[Cl-].[Ca+2].C1[N:41]([CH2:42][CH2:43][CH2:44]S(O)(=O)=O)CCOC1.[C:49]([OH:59])(=[O:58])C1C=CC(O)=C(O)C=1.[OH:60][C:61](CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:62].[OH-].[Na+]>[O-]S([O-])(=O)=O.[Cu+2]>[NH2:41][C@H:42]([C:49]([OH:59])=[O:58])[CH2:43][CH2:44][C:61]([OH:62])=[O:60]

Participants

11

Reactant 9

HCID 72Protocatechuic AcidHCID 14798Sodium HydroxideHCID 24450Potassium Phosphate, DibasicHCID 70807MopsHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 5284359Calcium ChlorideHCID 6097028Ammonium Sulfate

Product 1

HCID 33032L-Glutamic Acid

Reagents, catalysts & solvents 1

HCID 24462Copper SulfateCatalyst

Procedure

On the CM2B agar medium, the 2A-1R strain could form colonies at substantially the same rate as that of the wild-type strain ATCC13869. However, on the minimum plate medium (20 g/l glucose, 2.64 g/l ammonium sulfate, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 0.25 g/l MgSO4.7H2O, 0.01 g/l FeSO4.7H2O, 0.01 g/l MnSO4.7H2O, 0.01 g/l CaCl2, 0.02 mg/l CuSO4, 40 g/l MOPS, 30 mg/l protocatechuic acid, 200 μg/l VB1.Hu, 300 μg/l Biotin, 20 g/l agar, adjusted to pH 6.7 with NaOH), the 2A-1R strain showed a considerably decreased colony-forming rate as compared to the wild-type ATCC13869 strain. Accordingly, a gene that can recover the growth of the 2A-1R strain in the minimum medium was found.

Workup

  1. customOn the CM2B agar medium, the 2A-1R strain could form colonies at substantially the same rate as that of the wild-type strain ATCC13869
  2. customa considerably decreased colony-forming rate
  3. customAccordingly, a gene that can recover the growth of the 2A-1R strain in the minimum medium

Sources & evidence

ORD record
ord-4b893fa5b05d4628aae3bddcb32aaf81
Source dataset
uspto-grants-2012_10
Patent number
US08278074B2

Reaction notes

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