反应详情

温度

40 °C

In 5 mL of dimethyl sulfoxide is dissolved 0.42 g of L-alanyl-L-alanine trifluoroacetate. Then, 1.07 mL of triethylamine and 0.71 g of methyl 3-hydroxy-2-pyrazinecarboxylate are successively added to the solution, which is then stirred at 40° C. for 17 hours. The solvent is distilled off under reduced pressure, and 2 mL of water was added to the residue thus obtained. The deposited product is collected by filtration and purified by column chromatography [eluent: chloroform:methanol=30:1) to obtain 0.035 g of (2S)-2-[((2S)-2-{[(3-oxo-3,4-dihydro-2-pyrazinyl) carbonyl]amino}-propanoyl)amino]propanoic acid.

1. distillationThe solvent is distilled off under reduced pressure, and 2 mL of water
2. additionwas added to the residue
3. customthus obtained
4. filtrationThe deposited product is collected by filtration
5. custompurified by column chromatography [eluent