反应详情

To a stirred solution of 3-[5-cyano-2-oxo-3-[(phenylmethyl)amino]-1(2H)-pyrazinyl]-N-cyclopropyl-4-methyl-benzamide (Example 104, 100 mg) in methanol (1 mL) was added conc. ammonia solution (1 mL) followed by 30% hydrogen peroxide solution (1 mL). After 12 h, water was added and the mixture was extracted with dichloromethane. The pooled organics were washed with brine, dried (MgSO4), filtered and the solvent removed. Purification by preparative HPLC (Gemini column, 0.1% ammonia: acetonitrile eluent) afforded the title compound (31 mg).

1. extractionthe mixture was extracted with dichloromethane
2. washThe pooled organics were washed with brine
3. dry with materialdried (MgSO4)
4. filtrationfiltered
5. customthe solvent removed
6. customPurification by preparative HPLC (Gemini column, 0.1% ammonia: acetonitrile eluent)