Glycine → L-Ascorbic Acid

HRID 2073160Notes

Reaction equation

HRID 2073160 reaction equation
NCC(O)=O.[CH2:6]([OH:17])[C@H:7]([OH:16])[C@H:8]1[O:13][C:11](=[O:12])[C@@H:10]([OH:14])[C@@H:9]1[OH:15]>C(O)C>[O:12]=[C:11]1[O:13][C@H:8]([C@H:7]([CH2:6][OH:17])[OH:16])[C:9]([OH:15])=[C:10]1[OH:14]

Participants

4

Reactant 2

HCID 750Glycine2×HCID 6857365L-Galactono-1,4-lactone

Product 1

HCID 54670067L-Ascorbic Acid

Reagents, catalysts & solvents 1

HCID 702EthanolSolvent

Procedure

A 4.0 liter laboratory scale airlift fermentor was filled with sterile glycine medium, pH 4.1, containing 2.75% w/v ethanol, 0.7% glycine and 0.5% L-galactono-gamma-lactone. The fermentor was inoculated with a 24 hour suspension of the C. norvegensis KCC MF-42 cells washed from G-Agar (2.5%) flasks. The viable cell count at 0 hour was 5.5×106. Aeration of the fermentor was adjusted to 1.9 liters of air per liter of fermentation medium per minute [V/V/minute]which provided a cycle rate of 5.0 m-1. After 24 hours at 30° C., the viable cell count rose to 1.1×108, and at 48 hours was 3.0×108, at 72 hours the viable cell count was 2.8×108. The count declined to 1.7×108 after 91 hours of cultivation. A level of 0.72 gL-1 of L-ascorbic acid was produced.

Workup

  1. additioncontaining 2.75%
  2. additionsuspension of the C
  3. washnorvegensis KCC MF-42 cells washed from G-Agar (2.5%) flasks
  4. waitThe viable cell count at 0 hour was
  5. customV/V/minute]which provided a cycle rate of 5.0 m-1
  6. waitAfter 24 hours at 30° C.
  7. waitat 48 hours was
  8. wait3.0×108, at 72 hours the viable cell count was
  9. waitThe count declined to 1.7×108 after 91 hours of cultivation

Sources & evidence

ORD record
ord-d0ab1a1aecb2461e9c5cec0954899795
Source dataset
uspto-grants-1990_04
Patent number
US04916068

Reaction notes

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