Glycine → L-Ascorbic Acid

HRID 2073161Notes

Reaction equation

HRID 2073161 reaction equation
NCC(O)=O.[CH2:6]([OH:17])[C@H:7]([OH:16])[C@H:8]1[O:13][C:11](=[O:12])[C@@H:10]([OH:14])[C@@H:9]1[OH:15].O=O>C(O)C>[O:12]=[C:11]1[O:13][C@H:8]([C@H:7]([CH2:6][OH:17])[OH:16])[C:9]([OH:15])=[C:10]1[OH:14]

Participants

5

Reactant 3

HCID 750GlycineHCID 977Oxygen3×HCID 6857365L-Galactono-1,4-lactone

Product 1

HCID 54670067L-Ascorbic Acid

Reagents, catalysts & solvents 1

HCID 702EthanolSolvent

Procedure

In a similar airlift tower experiment a high cell density fermentation was performed. In this instance a 24 hour wet cell paste of C. norvegensis KCC MF-42 was dispersed in the 4.0 liter tower at a level of 100 gL-1 in SM-l medium containing 0.7% glycine and 0.7% L-Galactono-gamma-lactone. Ethanol was supplied continuously at a level (0.1-0.3%) neither limiting or inhibitory to yeast growth or productivity. Oxygen-enriched aeration was supplied to the fermentor at 1:1 oxygen-air ratio. Total mixed gas volume was 1.7 V/V/min. Under these conditions a dissolved oxygen saturation level of 30% was maintained in the upper section of the tower. After 20 hours of fermentation, L-ascorbic acid was produced at a level of 1.44 gL-1.

Workup

  1. customa 24 hour wet cell paste of C
  2. additionnorvegensis KCC MF-42 was dispersed in the 4.0 liter tower at a level of 100 gL-1 in SM-l medium
  3. additionTotal mixed gas volume

Sources & evidence

ORD record
ord-4ef0e927fed44af9baf189dba1082b71
Source dataset
uspto-grants-1990_04
Patent number
US04916068

Reaction notes

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