反应详情

The compound (209 mg) obtained in Example 1-8 was dissolved in anhydrous methanol (8.4 ml) and added with [1,4]-dioxan-2,5-diol (54.0 mg) and sodium cyanoborohydride (56.6 mg). Then, the solution was adjusted to pH 5 with acetic acid and stirred at room temperature for 19.5 hours. After completion of the reaction, the solvent was distilled off. The resultant was added with a 1 mol/l sodium hydroxide aqueous solution (1.0 ml) and subjected to extraction with chloroform, followed by drying with magnesium sulfate. The solvent was distilled off and the residue was then purified through silica gel column chromatography (chloroform/methanol) and treated with hydrochloric acid, thereby obtaining a hydrochloride (175.8 mg) of the subject compound as a white solid.

1. customAfter completion of the reaction
2. distillationthe solvent was distilled off
3. additionThe resultant was added with a 1 mol/l sodium hydroxide aqueous solution (1.0 ml)
4. extractionsubjected to extraction with chloroform
5. dry with materialby drying with magnesium sulfate
6. distillationThe solvent was distilled off
7. customthe residue was then purified through silica gel column chromatography (chloroform/methanol)
8. additiontreated with hydrochloric acid