Thiamine Hydrochloride → L-(-)-Ornithine

Participants

Reaction Steps

Media containing 10 g/dl of glucose, 7 g/dl of (NH4)2SO4, 0.15 g/dl of KH2PO4, 0.04 g/dl of MgSO4.7H2O, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 100 μg/1 of thiamine hydrochloride, 100 μg/1 of biotin, 60 mg of soybean protein acid hydrolysate (calculated as total nitrogen) and 15 mg/dl of L-arginine and 5 g/dl of calcium carbonate (independently sterilized) was adjusted to pH 7.2 and 25 ml of the media was charged into a 500 ml flask equipped with a shoulder followed by sterilization with heating. One platinum loop of the strain shown in Table 2 was inoculated on the medium and shaken for 4 days while keeping at 31.5° C. L-Ornithine was produced and accumulated in the culture solution of each strain in the amount shown in Table 2.

Provenance & Evidence