Thiamine Hydrochloride → L-(-)-Ornithine

HRID 2082281Notes

Reaction equation

HRID 2082281 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[Cl-].OC(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O.[NH2:55][C@H:56]([C:64]([OH:66])=[O:65])[CH2:57][CH2:58][CH2:59][NH:60]C(=N)N.C(=O)([O-])[O-].[Ca+2]>[Pt]>[NH2:55][C@H:56]([C:64]([OH:66])=[O:65])[CH2:57][CH2:58][CH2:59][NH2:60]

Participants

8

Reactant 6

HCID 6202Thiamine HydrochlorideHCID 10112Calcium CarbonateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 178418600HCID 178418600, compound

Product 1

HCID 6262L-(-)-Ornithine

Reagents, catalysts & solvents 1

HCID 23939PlatinumCatalyst

Procedure

Media containing 10 g/dl of glucose, 7 g/dl of (NH4)2SO4, 0.15 g/dl of KH2PO4, 0.04 g/dl of MgSO4.7H2O, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 100 μg/1 of thiamine hydrochloride, 100 μg/1 of biotin, 60 mg of soybean protein acid hydrolysate (calculated as total nitrogen) and 15 mg/dl of L-arginine and 5 g/dl of calcium carbonate (independently sterilized) was adjusted to pH 7.2 and 25 ml of the media was charged into a 500 ml flask equipped with a shoulder followed by sterilization with heating. One platinum loop of the strain shown in Table 2 was inoculated on the medium and shaken for 4 days while keeping at 31.5° C. L-Ornithine was produced and accumulated in the culture solution of each strain in the amount shown in Table 2.

Workup

  1. additionwas charged into a 500 ml flask
  2. customequipped with a shoulder
  3. temperaturewith heating
  4. customwhile keeping at 31.5° C

Sources & evidence

ORD record
ord-969633cebd45432db6099fea115a8777
Source dataset
uspto-grants-1993_02
Patent number
US05188947

Reaction notes

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