Phosphoric Acid → D-(-)-Fructose

Participants

Reaction Steps

The concentration of glucose isomerase produced in the culture broth was measured by the following method. 10 ml of the culture broth was centrifuged for 5 minutes at 10,000 G. The precipitated cells were charged into a test tube with an outside diameter of 15 mm containing 2.0 ml of a glucose isomerase reaction solution containing a 1/15 M phosphoric acid buffer (pH 7.0) and per liter of the phosphoric acid buffer, 400 g of glucose and 0.5 millimole of CoCl2.6H2O, and reacted at 70° C for 1 hour. Then, 2.0 ml of 5% perchloric acid was added to terminate the reaction. Fructose formed in the reaction mixture was determined by the carbazole-cysteine-sulfuric acid reaction method, and the concentration of glucose isomerase was calculated.

Provenance & Evidence