Phosphoric Acid → D-(-)-Fructose

HRID 2083564Notes

Reaction equation

HRID 2083564 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P(=O)(O)(O)O.Cl(O)(=O)(=O)=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

4

Reactant 3

HCID 1004Phosphoric Acid2×HCID 24247Perchloric AcidHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 1

HCID 5984D-(-)-Fructose

Procedure

The concentration of glucose isomerase produced in the culture broth was measured by the following method. 10 ml of the culture broth was centrifuged for 5 minutes at 10,000 G. The precipitated cells were charged into a test tube with an outside diameter of 15 mm containing 2.0 ml of a glucose isomerase reaction solution containing a 1/15 M phosphoric acid buffer (pH 7.0) and per liter of the phosphoric acid buffer, 400 g of glucose and 0.5 millimole of CoCl2.6H2O, and reacted at 70° C for 1 hour. Then, 2.0 ml of 5% perchloric acid was added to terminate the reaction. Fructose formed in the reaction mixture was determined by the carbazole-cysteine-sulfuric acid reaction method, and the concentration of glucose isomerase was calculated.

Workup

  1. additionThe precipitated cells were charged into a test tube with an outside diameter of 15 mm
  2. customreaction solution
  3. customto terminate
  4. customthe reaction

Sources & evidence

ORD record
ord-892073042e8f443cb77d81a8e2249dca
Source dataset
uspto-grants-1978_04
Patent number
US04086138

Reaction notes

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