Trifluoroacetic acid → 5-((S)-2-Pyrrolidin-2-yl-3H-imidazol-4-yl)-2-[4-((S)-2-pyrrolidin-2-yl-3H-imidazol-4-yl)-phenyl]-pyrimidine

HRID 20874Notes

Reaction equation

HRID 20874 reaction equation
C(O)(C(F)(F)F)=O.C(OC([N:15]1[CH2:19][CH2:18][CH2:17][C@H:16]1[C:20]1[N:21](COCC[Si](C)(C)C)[C:22]([C:25]2[CH:26]=[N:27][C:28]([C:31]3[CH:36]=[CH:35][C:34]([C:37]4[NH:38][C:39]([C@@H:42]5[CH2:46][CH2:45][CH2:44][N:43]5C(OC(C)(C)C)=O)=[N:40][CH:41]=4)=[CH:33][CH:32]=3)=[N:29][CH:30]=2)=[CH:23][N:24]=1)=O)(C)(C)C>C(Cl)Cl>[NH:15]1[CH2:19][CH2:18][CH2:17][C@H:16]1[C:20]1[NH:21][C:22]([C:25]2[CH:26]=[N:27][C:28]([C:31]3[CH:36]=[CH:35][C:34]([C:37]4[NH:38][C:39]([C@@H:42]5[CH2:46][CH2:45][CH2:44][NH:43]5)=[N:40][CH:41]=4)=[CH:33][CH:32]=3)=[N:29][CH:30]=2)=[CH:23][N:24]=1

Participants

4

Reactant 2

HCID 6422Trifluoroacetic acidHCID 24951274(S)-2-[5-(2-{4-[2-((S)-1-tert-Butoxycarbonyl-pyrrolidin-2-yl)-3H-imidazol-4-yl]-phenyl}-pyrimidin-5-yl)-1-(2-trimethylsilanyl-ethoxymethyl)-1H-imidazol-2-yl]-pyrrolidine-1-carboxylic acid tert-butyl ester

Product 1

HCID 249512755-((S)-2-Pyrrolidin-2-yl-3H-imidazol-4-yl)-2-[4-((S)-2-pyrrolidin-2-yl-3H-imidazol-4-yl)-phenyl]-pyrimidineYield 36.3%

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

TFA (8 mL) was added in one portion to a stirred solution of (S)-2-[5-(2-{4-[2-((S)-1-tert-butoxycarbonyl-pyrrolidin-2-yl)-3H-imidazol-4-yl]-phenyl}-pyrimidin-5-yl)-1-(2-trimethylsilanyl-ethoxymethyl)-1H-imidazol-2-yl]-pyrrolidine-1-carboxylic acid tert-butyl ester (1.50 g, 1.98 mmol) in dry CH2Cl2 (30 mL) at room temperature. The flask was sealed and the mixture was stirred at room temperature for 16 h before the solvent(s) were removed in vacuo. The residue was taken up in methanol, filtered through a PVDF syringe filter (13 mm×0.45 μm), distributed to 8 pHPLC vials and chromatographed by HPLC (gradient elution from 10% B to 100% B over 13 min on a Phenomenex C18 column, 30×100 mm, 10 μm, where A=90% water, 10% methanol, 0.1% TFA, B=10% water, 90% methanol, 0.1% TFA). After concentration of the selected test tubes by speed vacuum evaporation, the product was dissolved in methanol and neutralized by passing the solution through an UCT CHQAX 110M75 anion exchange cartridge. There was isolated the title compound as a yellow mustard-colored solid (306.7 mg, 36% yield) upon concentration of the eluant.

Workup

  1. customThe flask was sealed
  2. customwere removed in vacuo
  3. filtrationfiltered through a PVDF syringe
  4. filtrationfilter (13 mm×0.45 μm)
  5. customchromatographed by HPLC (
  6. washgradient elution from 10% B to 100% B over 13 min on a Phenomenex C18 column, 30×100 mm, 10 μm, where A=90% water, 10% methanol, 0.1% TFA, B=10% water, 90% methanol, 0.1% TFA)
  7. customAfter concentration of the selected test tubes by speed vacuum evaporation
  8. dissolutionthe product was dissolved in methanol

Sources & evidence

ORD record
ord-11d2b3071a814ed1a5a03b48f0ec9fde
Source dataset
uspto-grants-2010_06
Patent number
US07745636B2

Reaction notes

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