反应详情

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1 N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

1. additionwas added at room temperature
2. customwas obtained
3. customto obtain 400 ml final volume
4. filtrationThe solution was filtered through a 0.22-micron
5. filtrationfilter
6. additionto dilute the protein solution