反应详情

温度

25 °C

To a suspension of a mixture of 7-(aminomethyl)-6,7,8,9-tetrahydro-5H-benzo[7]annulen-1-amine and 7-(aminomethyl)-6,7,8,9-tetrahydro-5H-benzo[7]annulen-2-amine (300 mg, 1.577 mmol) in dichloromethane (5 ml) were added o-benzotriazol-1-yl-tetramethyluronium hexafluorophosphate (1315 mg, 3.47 mmol), 1-hydroxybenzotriazole (320 mg, 2.365 mmol), diisopropylethylamine (0.67 ml, 3.84 mmol), and 4-(5-cyano-7-isopropyl-1,3-benzoxazol-2-yl)benzoic acid (435 mg, 1.419 mmol, INTERMEDIATE 2), and the mixture was stirred overnight at 25° C. The residue was purified by flash chromatography on a Biotage Horizon, 40M column, eluting with 1 column volume of 100% dichloromethane followed by a gradient of 0 to 100% ethyl acetate in dichloromethane over 10 column volumes. The resulting mixture was repurified under the same conditions to provide the two diastereomers N-[(1-amino-6,7,8,9-tetrahydro-5H-benzo[7]annulen-7-yl)methyl]-4-(5-cyano-7-isopropyl-1,3-benzoxazol-2-yl)benzamide (90 mg, 0.188 mmol, 11.93% yield) and 1-(2-amino-6,7,8,9-tetrahydro-5H-benzo[7]annulen-7-yl)methanamine (56 mg, 0.117 mmol, 7.42% yield). Mass spectrum for N-[(1-amino-6,7,8,9-tetrahydro-5H-benzo[7]annulen-7-yl)methanamine (ESI) 479.2 (M+1). Mass spectrum for 1-(2-amino-6,7,8,9-tetrahydro-5H-benzo[7]annulen-7-yl)methanamine (ESI) 479.0 (M+1). 1H NMR data for the mixture (500 MHz, CDCl3): δ 8.32 (d, J=8.5 Hz, 2H), 7.90-8.96 (m, 4H), 7.51 (s, 1H), 7.49 (d, J=11 Hz, 1H), 7.49 (d, J=8.0 Hz, 2H), 7.32 (d, J=7.5 Hz, 2H), 7.25 (d, J=8.5 Hz, 1H), 7.20 (t, J=7.5 Hz, 1H), 6.42 (br s, 1H), 3.30-3.52 (m, 4H), 3.14 (dd, J=8.0, 14.0 Hz, 1H), 2.86-3.02 (m, 4H), 2.67 (dd, J=11.5, 14.5 Hz, 1H), 2.06-2.20 (m, 4H), 1.45 (d, J=7.5 Hz, 6H), 1.40-1.50 (m, 2H), 1.14-1.36 (m, 5H).

1. customThe residue was purified by flash chromatography on a Biotage Horizon, 40M column
2. washeluting with 1 column volume of 100% dichloromethane
3. customThe resulting mixture was repurified under the same conditions