Potassium Phosphate, Tribasic → HCID 44512165, compound

HRID 2091452Notes

Reaction equation

HRID 2091452 reaction equation
[NH2:1][CH2:2][CH2:3][NH:4][S:5]([C:8]1[CH:9]=[C:10]([CH:27]=[CH:28][CH:29]=1)[CH2:11][NH:12][C:13]([C:15]1[CH:16]=[N:17][C:18]([C:21]2[CH:26]=[CH:25][CH:24]=[CH:23][CH:22]=2)=[N:19][CH:20]=1)=[O:14])(=[O:7])=[O:6].P([O-])([O-])([O-])=O.[K+].[K+].[K+].[CH:38]1[C:43]([C:44](O)=[O:45])=[CH:42][C:41]2[C:47]([O:49][C:50]3([C:60]4[CH:61]=[CH:62][C:63]([OH:65])=[CH:64][C:59]=4[O:58][C:52]4[CH:53]=[C:54]([OH:57])[CH:55]=[CH:56][C:51]3=4)[C:40]=2[CH:39]=1)=[O:48]>CN(C)C=O>[OH:57][C:54]1[CH:53]=[C:52]2[C:51](=[CH:56][CH:55]=1)[C:50]([C:40]1[CH:39]=[CH:38][C:43]([C:44](=[O:45])[NH:1][CH2:2][CH2:3][NH:4][S:5]([C:8]3[CH:29]=[CH:28][CH:27]=[C:10]([CH2:11][NH:12][C:13]([C:15]4[CH:20]=[N:19][C:18]([C:21]5[CH:22]=[CH:23][CH:24]=[CH:25][CH:26]=5)=[N:17][CH:16]=4)=[O:14])[CH:9]=3)(=[O:6])=[O:7])=[CH:42][C:41]=1[C:47]([OH:49])=[O:48])=[C:60]1[C:59](=[CH:64][C:63](=[O:65])[CH:62]=[CH:61]1)[O:58]2

Participants

5

Reactant 3

HCID 62657Potassium Phosphate, TribasicHCID 1237555-FamHCID 57959392HCID 57959392, compound2×

Product 1

HCID 44512165HCID 44512165, compound

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent

Procedure

To a mixture consisting of N-(3-(N-(2-aminoethyl)sulfamoyl)benzyl)-2-phenylpyrimidine-5-carboxamide (Compound 19, 8.7 mg) in N,N-dimethylformamide (1 mL) was added 250 mM potassium phosphate buffer, pH 8 (2 mL) and 5-carboxyfluorescein, succinimidyl ester (5-FAM, SE; Biotium Catalog No. 90029; 10 mg). The mixture was stirred in the dark until the reaction was complete. The crude product was purified by preparative thin-layer chromatography (75:15:2 chloroform-methanol-water) to afford the title compound (approximately 4 mg); MS (ESI−) m/z 768 (M−1).

Workup

  1. customThe crude product was purified by preparative thin-layer chromatography (75:15:2 chloroform-methanol-water)

Sources & evidence

ORD record
ord-b5ad949bb5dd4932a8019d7cb44702df
Source dataset
uspto-grants-2013_05
Patent number
US08440417B2

Reaction notes

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