反应详情

温度

80 °C

2-[5-Bromo-6-oxo-4-{[(1R,2R,3R,5S)-2,6,6-trimethylbicyclo[3.1.1]hept-3-yl]amino}pyridazin-1(6H)-yl]-N-[(2-cyanopyridin-4-yl)methyl]acetamide (34.0 mg, 0.07 mmol) was dissolved in ethanol/30% aqueous potassium hydroxide (4 mL, 1:1) and stirred at 80° C. for 2 hours. After cooling, ethyl acetate was added, and the organic layer was washed with saturated aqueous ammonium chloride, dried over anhydrous sodium sulfate and evaporated under reduced pressure. The resulting residue was purified by silica gel column chromatography (ethyl acetate/methanol=8/1) to give the desired product.

1. temperatureAfter cooling
2. washthe organic layer was washed with saturated aqueous ammonium chloride
3. dry with materialdried over anhydrous sodium sulfate
4. customevaporated under reduced pressure
5. customThe resulting residue was purified by silica gel column chromatography (ethyl acetate/methanol=8/1)