反应详情

The following solutions were prepared: 1 μL of 1M potassium ferricyanide aqueous solution obtained by dissolving potassium ferricyanide (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 6.7 μL of 1000 U/mL diaphorase solution obtained by dissolving diaphorase (manufactured by UNITIKA LTD.) in 50 mM phosphate buffer (pH 7.5); 7.8 μL of 4M sodium malate aqueous solution obtained by dissolving sodium malate (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 7.8 μL of 4M malic acid aqueous solution obtained by dissolving malic acid (manufactured by Wako Pure Chemical Industries, Ltd.) in pure water; 7.8 μL of 4M potassium malate aqueous solution obtained by dissolving potassium malate (manufactured by KANTO CHEMICAL CO., INC.) in pure water; 1 μL of 5 mM NADP aqueous solution obtained by dissolving NADP (manufactured by ORIENTAL YEAST Co., Ltd.) in pure water; and 1 μL of 25000 U/mL malate dehydrogenase solution containing malate dehydrogenase dissolved in 50% of glycerol (manufactured by Roche Diagnostics, Inc.).