NADP nicotinamide-adenine-dinucleotide phosphate → NADPH
Reaction equation
C(O)C(N)(CO)CO.Cl.[CH:10]1[CH:15]=[N+:14]([C@@H:16]2[O:20][C@H:19]([CH2:21][O:22][P:23]([O:26][P:27]([O:30][CH2:31][C@H:32]3[O:36][C@@H:35]([N:37]4[C:41]5[N:42]=[CH:43][N:44]=[C:45]([NH2:46])[C:40]=5[N:39]=[CH:38]4)[C@H:34]([O:47][P:48]([OH:51])([OH:50])=[O:49])[C@@H:33]3[OH:52])([OH:29])=[O:28])([OH:25])=[O:24])[C@@H:18]([OH:53])[C@H:17]2[OH:54])[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH:11]=1.[Cl-].[Mg+2].[Cl-].P(OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O)(O)(O)=O>>[CH:43]1[N:44]=[C:45]([NH2:46])[C:40]2[N:39]=[CH:38][N:37]([C@@H:35]3[O:36][C@H:32]([CH2:31][O:30][P:27]([O:26][P:23]([O:22][CH2:21][C@H:19]4[O:20][C@@H:16]([N:14]5[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH2:11][CH:10]=[CH:15]5)[C@H:17]([OH:54])[C@@H:18]4[OH:53])([OH:25])=[O:24])([OH:29])=[O:28])[C@@H:33]([OH:52])[C@H:34]3[O:47][P:48]([OH:51])([OH:50])=[O:49])[C:41]=2[N:42]=1Participants
5
Procedure
The crude enzyme solution was added to a reaction mixture comprising 50 mmol/l Tris/HCl (pH 7.5), 0.5 mmol/l NADP, 10 mmol/l magnesium chloride and 2 mmol/l 6-phosphogluconic acid to make up to 1.5 ml. The resulting mixture was subjected to reaction in a 1-ml cuvette at 30° C. and the increase in the absorbance was measured at 340 nm. On the basis of the molar extinction coefficient of NADPH (6220), the amount of NADPH formed per minute was calculated. GND specific activity was expressed in terms of the amount of NADPH formed per mg of protein per minute.
Workup
- customThe resulting mixture was subjected to reaction in a 1-ml cuvette at 30° C.
- temperaturethe increase in the absorbance
Sources & evidence
- ORD record
- ord-227137d2213e40a0a2cd575eb061b7ec
- Source dataset
- uspto-grants-2009_04
- Patent number
- US07524667B2
Reaction notes
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