反应详情

(2R,4R)-5-Biphenyl-4-yl-4-t-butoxycarbonylamino-2-hydroxypentanoic acid (30 mg, 78 μmol), HOBt (63 mg, 470 μmol), and EDCI (83 μL, 470 μmol) were dissolved into DCM. After stirring for 10 minutes, 4-nitrooxybutan-1-ol (84.1 mg, 623 μmol) was added. The resulting mixture was stirred at room temperature and the reaction monitored for completion (˜30 minutes). The mixture was stirred for an additional 2 hours and then left open to dry. The product was then purified (Interchim reverse phase chromatography column; 35-95% MeCN in water with 0.05% TFA) to yield the title compound (18 mg).

1. stirringThe resulting mixture was stirred at room temperature
2. custommonitored for completion (˜30 minutes)
3. stirringThe mixture was stirred for an additional 2 hours
4. waitleft open
5. customto dry
6. customThe product was then purified (Interchim reverse phase chromatography column; 35-95% MeCN in water with 0.05% TFA)