7-Amino-4-methylcoumarin → (-)-Asparagine

HRID 2170067Notes

Reaction equation

HRID 2170067 reaction equation
CC([O-])=O.[Na+].N(C(OCC1C=CC=CC=1)=O)[C@H](C(N[C@H](C([NH:16][C@H:17]([C:22]([OH:24])=[O:23])[CH2:18][C:19](=O)[OH:20])=O)C)=O)C.[NH2:35]C1C=C2C(C(C)=CC(=O)O2)=CC=1>>[NH2:16][C@H:17]([C:22]([OH:24])=[O:23])[CH2:18][C:19](=[O:20])[NH2:35]

Participants

4

Reactant 3

HCID 922497-Amino-4-methylcoumarinHCID 517045Sodium AcetateHCID 131732930HCID 131732930, compound

Product 1

HCID 6267(-)-Asparagine

Procedure

To a AEP (100 ng/well) in assay buffer (50 mM NaOAc, 300 mM NaCL, pH 4.5, 50 μL) was added 10 μL of the inhibitors (in PBS). The plate was then incubated at room temperature for 20 minutes. ZAla-Ala-Asp-MEC was then added (100 μM, Bachem, 60 μL) and 7-amino-4-methylcoumarin release was measured by fluorescence spectroscopy over time. Initial rates were plotted against inhibitor concentration.10

Workup

  1. concentrationInitial rates were plotted against inhibitor concentration.10

Sources & evidence

ORD record
ord-9c4bd092cea84f3599d41e9461af2749
Source dataset
uspto-grants-2015_06
Patent number
US09050374B2

Reaction notes

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