7-Amino-4-methylcoumarin → (-)-Asparagine

Participants

Reaction Steps

To a AEP (100 ng/well) in assay buffer (50 mM NaOAc, 300 mM NaCL, pH 4.5, 50 μL) was added 10 μL of the inhibitors (in PBS). The plate was then incubated at room temperature for 20 minutes. ZAla-Ala-Asp-MEC was then added (100 μM, Bachem, 60 μL) and 7-amino-4-methylcoumarin release was measured by fluorescence spectroscopy over time. Initial rates were plotted against inhibitor concentration.10

Provenance & Evidence