Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Propylene Glycol | HCID 1030 | — |
| Reactant | Hydroxyacetone | HCID 8299 | — |
| Reactant | Mops | HCID 70807 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | Propylene Glycol | HCID 1030 | — |
| Product | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | HCID 86646416 | HCID 86646416 | — |
Reaction Steps
The strains obtained as described in example 4 (strains 1, 2 and 3) and the control strains (control 1: MG1655 pME101VB01-yqhD-mgsA-gldA, control 2: MG1655 pME101VB01-yafB-mgsA-gldA, control 3: MG1655 pME101VB01-yqhE-mgsA-gldA and control 4: MG1655 Ptrc16-gapA, Δedd-eda, ΔgloA, ΔpykA, ΔpykF) were cultivated in an Erlenmeyer flask assay under aerobic conditions in minimal medium with glucose as carbon source. The culture was carried out at 34° C. or 37° C. and the pH was maintained by buffering the culture medium with MOPS. At the end of the culture, 1,2-propanediol, acetol and residual glucose in the fermentation broth were analysed by HPLC and the yields of 1,2-propanediol over glucose and 1,2-propanediol+acetol over glucose were calculated.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US09051588B2 |
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| Source dataset | uspto-grants-2015_06 |
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| ORD ID | ord-3e0199d975464b2183720d0b53169437 |
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| Source note | uspto-grants-2015_06 |
|---|
