Propylene Glycol → Propylene Glycol

HRID 2172374Notes

Reaction equation

HRID 2172374 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].C1N(CCCS(O)(=O)=O)CCOC1.[CH2:26]([OH:30])[CH:27]([OH:29])[CH3:28].[CH3:31][C:32]([CH2:34][OH:35])=[O:33]>>[CH2:2]([OH:1])[CH:3]([OH:4])[CH3:5].[CH2:26]([OH:30])[CH:27]([OH:29])[CH3:28].[CH3:31][C:32]([CH2:34][OH:35])=[O:33].[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4]

Participants

7

Reactant 4

HCID 1030Propylene GlycolHCID 8299HydroxyacetoneHCID 70807MopsHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 3

HCID 1030Propylene GlycolHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 86646416HCID 86646416, compound

Procedure

The strains obtained as described in example 4 (strains 1, 2 and 3) and the control strains (control 1: MG1655 pME101VB01-yqhD-mgsA-gldA, control 2: MG1655 pME101VB01-yafB-mgsA-gldA, control 3: MG1655 pME101VB01-yqhE-mgsA-gldA and control 4: MG1655 Ptrc16-gapA, Δedd-eda, ΔgloA, ΔpykA, ΔpykF) were cultivated in an Erlenmeyer flask assay under aerobic conditions in minimal medium with glucose as carbon source. The culture was carried out at 34° C. or 37° C. and the pH was maintained by buffering the culture medium with MOPS. At the end of the culture, 1,2-propanediol, acetol and residual glucose in the fermentation broth were analysed by HPLC and the yields of 1,2-propanediol over glucose and 1,2-propanediol+acetol over glucose were calculated.

Workup

  1. customThe strains obtained
  2. customwas carried out at 34° C. or 37° C.
  3. temperaturethe pH was maintained

Sources & evidence

ORD record
ord-3e0199d975464b2183720d0b53169437
Source dataset
uspto-grants-2015_06
Patent number
US09051588B2

Reaction notes

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