Ethylenediaminetetraacetic Acid → Acridan

HRID 2182184Notes

Reaction equation

HRID 2182184 reaction equation
[C:1]1([C:7]2[CH:12]=[CH:11][C:10](O)=[CH:9][CH:8]=2)[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[C:14]([NH2:17])(N)=O.OO.C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O>>[CH:2]1[C:1]2[CH2:7][C:8]3[C:14](=[CH:12][CH:11]=[CH:10][CH:9]=3)[NH:17][C:6]=2[CH:5]=[CH:4][CH:3]=1

Participants

4

Reactant 3

HCID 6049Ethylenediaminetetraacetic AcidHCID 71034-HydroxybiphenylHCID 31294Carbamide Peroxide

Product 1

HCID 7106Acridan

Procedure

Chemiluminescent HRP detection limit using a reagent composition containing 0.05 mM 2′,3′,6′-trifluorophenyl 3-methoxy-10-methylacridan-9-carboxylate (compound 13), 0.1 mM 4-phenylphenol, 0.5 mM urea peroxide, 1 mM EDTA, and 0.025% TWEEN 20 in 0.01 M tris buffer, pH 8.0 was obtained on a Labsystems Luminoskan microtiter plate reader. White Microlite 1 FB 12-well strips (Dynatech Laboratories) containing 100 μL of the reagent in each well were reacted with 10 μL of HRP dilutions. Light intensity was measured at 10 min.

Workup

  1. customwas obtained on a Labsystems Luminoskan microtiter plate reader
  2. customwere reacted with 10 μL of HRP dilutions

Sources & evidence

ORD record
ord-f5b6c46e0ec84705adee90ba396e3032
Source dataset
uspto-grants-2005_05
Patent number
US06897036B2

Reaction notes

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