Phosphate → D-(-)-Fructose

HRID 2182185Notes

Reaction equation

HRID 2182185 reaction equation
P([O-])([O-])([O-])=O.[O:6]=[CH:7][C@H:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11])[OH:9]>O>[OH:6][CH2:7][C:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11])=[O:9]

Participants

4

Reactant 2

HCID 1061PhosphateHCID 161658Demannose

Product 1

HCID 5984D-(-)-Fructose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

To 0.5 ml of a 0.1M phosphate buffer (pH 7.0) containing 0.2M mannose, an appropriate amount of the enzyme was added, after which water was added to make the total volume 1.0 ml, and reacted at a temperature of 50° C. After the reaction was terminated by the addition of a 0.5M perchloric acid solution, the amount of fructose produced was determined by the cysteine-carbazole process. The amount of the enzyme, which produces one micromole of fructose in one minute under the above-described conditions is defined as one unit.

Workup

  1. additionan appropriate amount of the enzyme was added
  2. customreacted at a temperature of 50° C
  3. customAfter the reaction was terminated by the addition of a 0.5M perchloric acid solution

Sources & evidence

ORD record
ord-c9dc4cfdbdff4083adfc038464d8d543
Source dataset
uspto-grants-2005_05
Patent number
US06897047B1

Reaction notes

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