Phosphate → D-(-)-Fructose

Participants

Reaction Steps

To 0.5 ml of a 0.1M phosphate buffer (pH 7.0) containing 0.2M mannose, an appropriate amount of the enzyme was added, after which water was added to make the total volume 1.0 ml, and reacted at a temperature of 50° C. After the reaction was terminated by the addition of a 0.5M perchloric acid solution, the amount of fructose produced was determined by the cysteine-carbazole process. The amount of the enzyme, which produces one micromole of fructose in one minute under the above-described conditions is defined as one unit.

Provenance & Evidence