Phosphate → D-(-)-Fructose

HRID 2182186Notes

Reaction equation

HRID 2182186 reaction equation
NCC(O)=O.[OH-].[Na+].P([O-])([O-])([O-])=O.[O:13]=[CH:14][C@H:15]([C@H:17]([C@@H:19]([C@@H:21]([CH2:23][OH:24])[OH:22])[OH:20])[OH:18])[OH:16]>C(O)(=O)C>[OH:13][CH2:14][C:15]([C@H:17]([C@@H:19]([C@@H:21]([CH2:23][OH:24])[OH:22])[OH:20])[OH:18])=[O:16]

Participants

5

Reactant 3

HCID 1061PhosphateHCID 161658Demannose2×HCID 86654294Glycine sodium hydroxide

Product 1

HCID 5984D-(-)-Fructose

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

50 mm each of acetic acid buffer (pH 4.5 to 6.0), tris-HCl buffer (pH 7 to 8.6), glycine-NaOH buffer (pH 8.0 to 10.5) and phosphate buffer (Na2HPO4—NaOH, pH 8 to 11), 0.1M of mannose and 0.15 units of mannose isomerase were added to form a mixture with a total volume of 0.4 ml, and reacted at 40° C. for 30 minutes. The amount of fructose produced was determined by the cysteine-carbazole process. The relative activities at each pHs are shown in Table 3 and FIG. 1, with the activity at pH 8.2, which was the maximum activity, defined as 100.

Workup

  1. customreacted at 40° C. for 30 minutes

Sources & evidence

ORD record
ord-814223e8d5a04222938e88bb2e327229
Source dataset
uspto-grants-2005_05
Patent number
US06897047B1

Reaction notes

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