Phosphate → D-(-)-Fructose

Participants

Reaction Steps

50 mm each of acetic acid buffer (pH 4.5 to 6.0), tris-HCl buffer (pH 7 to 8.6), glycine-NaOH buffer (pH 8.0 to 10.5) and phosphate buffer (Na2HPO4—NaOH, pH 8 to 11), 0.1M of mannose and 0.15 units of mannose isomerase were added to form a mixture with a total volume of 0.4 ml, and reacted at 40° C. for 30 minutes. The amount of fructose produced was determined by the cysteine-carbazole process. The relative activities at each pHs are shown in Table 3 and FIG. 1, with the activity at pH 8.2, which was the maximum activity, defined as 100.

Provenance & Evidence