反应详情

To a solution of 2-[4-(4-acetyl-piperazin-1-yl)-phenylamino]-7-(1-ethyl-propyl)-7H-pyrrolo[2,3-d]pyrimidine-6-carboxylic acid methyl ester (19 mg, 0.041 mmol) in MeOH (1.5 mL) is added 2 N LiOH aqueous solution (0.5 mL). The reaction mixture is stirred overnight and concentrated in vacuo. The residue is purified by preparative HPLC to give 13.6 mg of 2-[4-(4-Acetyl-piperazin-1-yl)-phenylamino]-7-(1-ethyl-propyl)-7H-pyrrolo[2,3-d]pyrimidine-6-carboxylic acid.

1. concentrationconcentrated in vacuo
2. customThe residue is purified by preparative HPLC