反应详情

A solution of 1,1-dimethylethyl 3-[5-(5-{3-chloro-4-[(cyclopropylmethyl)oxy]phenyl}-1,2,4-oxadiazol-3-yl)-1-isoquinolinyl]propanoate (D45; 22 mg, 0.043 mmol) in 4N HCl in 1,4-dioxane (4 ml) was allowed to stand at room temperature for 2 h. The solvent was evaporated to give a white solid, which was triturated with dry ether to give a white solid (15 mg). The sample was dissolved in NMP (0.5 ml) and purified by Mass Directed Preparative HPLC (Supelcosil ABZ+Plus column, eluting with solvents A/B (A: Water+0.1% Formic acid, B: MeCN:Water 95:5+0.05% Formic acid)). The solvent was evaporated in vacuo to give the title compound (5 mg).

1. customThe solvent was evaporated
2. customto give a white solid, which
3. customwas triturated with dry ether