反应详情

1,1-Dimethylethyl 3-[5-(5-{3-chloro-4-[(difluoromethyl)oxy]phenyl}-1,2,4-oxadiazol-3-yl)-1-isoquinolinyl]propanoate (D46; 32 mg, 0.064 mmol) was dissolved in 4M hydrogen chloride in 1,4-dioxane, and the solution allowed to stand at room temperature for 2 h. The solvent was evaporated in vacuo to give a white solid which was triturated under dry ether (3×5 ml) to give a white solid. The sample was dissolved in 1:1 MeOH:DMSO (1 ml) and purified by Mass Directed Preparative HPLC (Supelcosil ABZ+Plus column, eluting with solvents NB (A: Water+0.1% Formic acid, B: MeCN:Water 95:5+0.05% Formic acid)). The solvent was evaporated in vacuo to give the title compound as a white solid (12.8 mg)

1. customThe solvent was evaporated in vacuo
2. customto give a white solid which
3. customwas triturated under dry ether (3×5 ml)
4. customto give a white solid
5. customDMSO (1 ml) and purified by Mass Directed Preparative HPLC (Supelcosil ABZ+Plus column
6. washeluting with solvents NB (A: Water+0.1% Formic acid, B
7. customThe solvent was evaporated in vacuo