反应详情

1,1-Dimethylethyl 3-(5-{5-[3-cyano-4-(3-fluoro-1-pyrrolidinyl)phenyl]-1,2,4-oxadiazol-3-yl}-1-isoquinolinyl)propanoate (D51; 14 mg, 0.027 mmol) was stirred in 4M HCl in dioxane (1 ml) at room temperature for 7 hrs. The mixture was concentrated in a blow down unit and the residue was purified by Mass Directed Preparative HPLC (Supelcosil ABZ+Plus column, eluting with solvents A/B (A: Water+0.1% Formic acid, B: MeCN:Water 95:5+0.05% Formic acid)) to give the title compound as a white solid (2 mg).

1. concentrationThe mixture was concentrated in a blow down unit
2. customthe residue was purified by Mass Directed Preparative HPLC (Supelcosil ABZ+Plus column
3. washeluting with solvents A/B (A: Water+0.1% Formic acid, B