反应详情

温度

22 °C

Charge a 500 mL round bottom 3-neck flask that equipped with a thermocouple, mechanical stirrer, nitrogen inlet/outlet and cooling bath with 202.8 g (0.622 mol) of cesium carbonate and 260 g (260 mL) of water. Stir and cool the resulting solution to 22±3° C. Transfer the solution to the addition funnel. Charge a nitrogen-flushed 3 L reactor that equipped with an overhead stirrer, condenser, pH probe, nitrogen inlet/outlet and 500 mL addition funnel with 50.0 g (0.207 mol) of 5-bromo-4-(trifluoromethyl) pyridin-2-amine 4a, 190.9 g (0.456 mol) of 4,4′[6(4,4,5,5tetramethyl1,3,2 dioxaborolan2yl)pyrimidine2,4diyl]di[morpholine] 4, 6.75 g (0.0103 mol) of 1,1′-bis(di-tert-butylphosphino) ferrocene palladium dichloride and 556 g (625 mL) of tetrahydrofuran. Stir the slurry at 22±3° C. Add the aqueous cesium carbonate solution via the addition funnel to the slurry over 1-2 min. Stir rapidly (to ensure good mixing), heat to 45±3° C. over 15 min and hold at this temperature for at least 30 minutes. Check for completeness of the reaction. Cool to 22±3° C. Separate the phases. Partially concentrate the THF (25 C, 90 mbar) to a volume of 400 mL. Add 654 g (750 mL) of isopropyl acetate, resume the vacuum distillation and concentrate to a volume of 400 mL. Add 610 g (700 mL) of isopropyl acetate, stir and filter the hazy solution through a 25 g pad of Celite. Wash the reactor and filter cake with 87 g (100 mL) of isopropyl acetate and add the wash to the batch. Add 1 L of 0.125N aqueous N-acetyl-L-cysteine solution and stir at 60±3° C. for 1 h. Cool to 22±3° C. C and drain the aqueous wash. Add 1 L of 0.25N aqueous N-acetyl-L-cysteine pH=7 solution and stir at 60±3° C. for 1 h. Cool to 22±3° C. and drain the aqueous wash. Again, add 1 L of 0.25N aqueous N-acetyl-L-cysteine pH=7 solution and stir at 60±3° C. for 1 h. Cool to 22±3° C. and drain the aqueous wash. Charge 34.5 g of Si-Thiol functionalized silica gel and stir the suspension at 60±3° C. for 1 h. Cool to 22±3° C. and filter to remove the silica gel. Add 1 L of 1N aqueous hydrochloric acid solution and stir for 15 minutes. Separate the phases and retain the aqueous phase which now contains product. Extract the organic phase again by adding 500 mL of 1N aqueous hydrochloric acid solution and stirring for 15 minutes. Separate the phases and combine the aqueous extracts. Adjust the pH to 2.3±0.2 by the addition of ˜280 mL of 4N aqueous sodium hydroxide solution. Charge 17.2 g of Si-Thiol functionalized silica gel and stir the suspension at 50±3° C. for 1 h. Cool to 22±3° C. and filter to remove the silica gel. Adjust the pH to 5.0±0.2 by the slow addition of ˜75 mL of 4N aqueous sodium hydroxide solution maintaining a batch temperature of 15±3° C. Stir the slurry for at least 16 h at 22±3° C. to allow the product to completely solidify. Filter the solids and wash the filter cake once with 250 g (250 mL) of water. Dry the solids (50° C., 35 mbar) for 16 h to obtain 75 g (89% yield) of 5 as a tan solid. Following this procedure, Compound 5 is the hemihydrate polymorph form HA of the Compound of Formula A.

1. additionCharge a 500 mL round bottom 3-neck flask
2. customthat equipped with a thermocouple, mechanical stirrer, nitrogen inlet/outlet
3. additionCharge a nitrogen-
4. customflushed 3 L reactor
5. customthat equipped with an overhead stirrer, condenser
6. stirringStir the slurry at 22±3° C
7. stirringStir rapidly
8. addition(to ensure good mixing)
9. temperatureheat to 45±3° C. over 15 min
10. waithold at this temperature for at least 30 minutes
11. customCheck for completeness of the reaction
12. temperatureCool to 22±3° C
13. customSeparate the phases
14. concentrationPartially concentrate the THF (25 C, 90 mbar) to a volume of 400 mL
15. additionAdd 654 g (750 mL) of isopropyl acetate
16. distillationthe vacuum distillation
17. concentrationconcentrate to a volume of 400 mL
18. additionAdd 610 g (700 mL) of isopropyl acetate
19. stirringstir
20. filtrationfilter the hazy solution through a 25 g pad of Celite
21. washWash the reactor
22. filtrationfilter cake with 87 g (100 mL) of isopropyl acetate
23. additionadd the
24. washwash to the batch
25. additionAdd 1 L of 0.125N aqueous N-acetyl-L-cysteine solution
26. stirringstir at 60±3° C. for 1 h
27. temperatureCool to 22±3° C
28. additionAdd 1 L of 0.25N aqueous N-acetyl-L-cysteine pH=7 solution
29. stirringstir at 60±3° C. for 1 h
30. temperatureCool to 22±3° C.
31. additionAgain, add 1 L of 0.25N aqueous N-acetyl-L-cysteine pH=7 solution
32. stirringstir at 60±3° C. for 1 h
33. temperatureCool to 22±3° C.
34. additionCharge 34.5 g of Si-Thiol functionalized silica gel
35. stirringstir the suspension at 60±3° C. for 1 h
36. temperatureCool to 22±3° C.
37. filtrationfilter
38. customto remove the silica gel
39. additionAdd 1 L of 1N aqueous hydrochloric acid solution
40. stirringstir for 15 minutes
41. customSeparate the phases
42. extractionExtract the organic phase again
43. additionby adding 500 mL of 1N aqueous hydrochloric acid solution
44. stirringstirring for 15 minutes
45. customSeparate the phases
46. additionby the addition of ˜280 mL of 4N aqueous sodium hydroxide solution
47. additionCharge 17.2 g of Si-Thiol functionalized silica gel
48. stirringstir the suspension at 50±3° C. for 1 h
49. temperatureCool to 22±3° C.
50. filtrationfilter
51. customto remove the silica gel
52. additionby the slow addition of ˜75 mL of 4N aqueous sodium hydroxide solution
53. temperaturemaintaining a batch temperature of 15±3° C
54. stirringStir the slurry for at least 16 h at 22±3° C.
55. filtrationFilter the solids
56. washwash the filter cake once with 250 g (250 mL) of water
57. customDry the solids (50° C., 35 mbar) for 16 h