反应详情

Add 4 M HCl in 1,4-dioxane (5 mL) to a solution of 3′-(2-fluoro-3-trifluoromethyl-phenyl)-2,3,5,6-tetrahydro-[1,2′]bipyrazinyl-4-carboxylic acid t-butyl ester (0.101 g, 0.237 mmol) in DCM (10 mL) and stir for 19 hr. Concentrate, dissolve the residue in methanol (20 mL) and purify by SCX chromatography (pre-wash column with methanol), load material, elute with 2 M ammonia in methanol, and concentrate to give the title preparation as a yellow solid (0.0985 g). MS (ES): m/z=327 [M+H]+.

1. concentrationConcentrate
2. dissolutiondissolve the residue in methanol (20 mL)
3. custompurify by SCX chromatography (pre-wash column with methanol), load material
4. washelute with 2 M ammonia in methanol
5. concentrationconcentrate
6. customto give the title