Hydrazine hydrate (1:1) → HCID 118207742, compound

HRID 2205620Notes

Reaction equation

HRID 2205620 reaction equation
[NH2:1][C:2]1[N:7]=[C:6]([NH:8][C@H:9]([C:11]2[N:16]=[C:15]3[CH:17]=[CH:18][N:19]([CH3:20])[C:14]3=[CH:13][C:12]=2[N:21]2[CH2:25][CH2:24][CH:23]([N:26]3C(=O)C4C(=CC=CC=4)C3=O)[CH2:22]2)[CH3:10])[C:5]([C:37]#[N:38])=[C:4]([CH3:39])[N:3]=1.O.NN>C(O)C.C(#N)C>[NH2:1][C:2]1[N:7]=[C:6]([NH:8][C@H:9]([C:11]2[N:16]=[C:15]3[CH:17]=[CH:18][N:19]([CH3:20])[C:14]3=[CH:13][C:12]=2[N:21]2[CH2:25][CH2:24][C@@H:23]([NH2:26])[CH2:22]2)[CH3:10])[C:5]([C:37]#[N:38])=[C:4]([CH3:39])[N:3]=1

Participants

5

Reactant 2

HCID 24654Hydrazine hydrate (1:1)HCID 118207749HCID 118207749, compound

Product 1

HCID 118207742HCID 118207742, compoundYield 31%

Reagents, catalysts & solvents 2

HCID 702EthanolSolventHCID 6342AcetonitrileSolvent

Conditions

Temperature
65 °C

Procedure

To a 25 mL pear flask was added 2-amino-4-(((S)-1-(6-(3-(1,3-dioxoisoindolin-2-yl)pyrrolidin-1-yl)-1-methyl-1H-pyrrolo[3,2-b]pyridin-5-yl)ethyl)amino)-6-methylpyrimidine-5-carbonitrile (peak 1 of PREPARATION 13, 27 mg, 0.052 mmol) in ethanol (520 μL) to give a solution. To this solution was added hydrazine monohydrate (25 μL, 0.52 mmol). The mixture was heated to 65° C. for 3.5 hours, at which time the reaction was deemed to be complete based on MS/UPLC. The mixture was diluted with acetonitrile and was directly purified by preparative HPLC (basic mode, 25-45% ACN/water gradient). The product-containing fractions were concentration and lyophilized to give the title compound as a white solid (6.3 mg, 31%). The absolute configuration of the stereocenter on the pyrrolidine ring was not determined, but was assigned the opposite configuration of EXAMPLE 8. 1H NMR (400 MHz, DMSO-d6) δ ppm 1.41 (d, J=6.32 Hz, 3H), 1.63-1.74 (m, 1H), 2.16 (dd, J=12.25, 7.20 Hz, 1H), 2.25 (s, 3H), 2.71 (dd, J=9.09, 4.80 Hz, 1H), 3.04 (td, J=8.27, 5.18 Hz, 1H), 3.60 (t, J=6.32 Hz, 1H), 3.71-3.85 (m, 3H), 5.75 (d, J=6.82 Hz, 1H), 6.42-6.51 (m, 1H), 7.18 (d, J=7.58 Hz, 1H), 7.51 (d, J=3.28 Hz, 1H), 7.68-7.78 (m, 1H); ESI-MS m/z [M+H]+ calc'd for C20H25N9, 392. found 392.

Workup

  1. customto give a solution
  2. customwas directly purified by preparative HPLC (basic mode, 25-45% ACN/water gradient)
  3. concentrationconcentration

Sources & evidence

ORD record
ord-8eaf92009b3f42b4a0f0000cc200654a
Source dataset
uspto-grants-2016_06
Patent number
US09371321B2

Reaction notes

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