反应详情

温度

70 °C

A mixture of methyl 3-(ethyl(4-hydroxy-4-methylcyclohexyl)amino)-2-methylbenzoate (300 mg, 0.98 mmol) and NaOH (58 mg, 1.47 mmol) in 5 ml of ethanol and water (3:2) was heated at 70° C. for 2 h. The reaction mixture was concentrated to dryness and the residue was partitioned between water and ethyl acetate. The organic layer was dried over Na2SO4 and concentrated under reduced pressure to afford 270 mg of the crude acid. The crude acid (270 g, 0.92 mmol), 3-(aminomethyl)-4,6-dimethylpyridin-2(1H)-one (282 mg, 1.85 mmol), PyBOP (717 mg, 1.38 mmol) and triethylamine (0.12 ml, 0.92 mmol) were stirred in 3 ml of DMSO at room temperature overnight. The reaction mixture was diluted with water and extracted with 10% MeOH in DCM. The combined organic phases were dried over Na2SO4, concentrated and the residue purified by silica gel column chromatography to give the title compound N-((4,6-dimethyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-3-(ethyl(4-hydroxy-4-methylcyclohexyl)amino)-2-methylbenzamide as a mixture of isomers (120 mg, 30%). LCMS: 426.45 (M+1)+; HPLC: 62.09 & 33.24% (@ 210-370 nm) (Rt; 3.982 & 4.164; Method: Column: YMC ODS-A 150 mm×4.6 mm×5μ; Mobile Phase: A; 0.05% TFA in water/B; 0.05% TFA in acetonitrile; Inj. Vol: 10 μL, Col. Temp.: 30° C.; Flow rate: 1.4 mL/min.; Gradient: 5% B to 95% B in 8 min, Hold for 1.5 min, 9.51-12 min 5% B); 1H NMR (DMSO-d6, 400 MHz) δ 11.44 (s, 1H), 8.04 (m, 1H), 7.12 (s, 2H), 6.91 (m, 1H), 5.85 (s, 1H), 4.25 (d, 2H, J=4 Hz), 4.15 (m, 1H), 3.00 (m, 2H), 2.84 (m, 1H), 2.18 (s, 6H), 2.10 (s, 3H), 1.75-1.60 (m, 2H), 1.55-1.35 (m, 4H), 1.35-1.15 (m, 4H), 1.08 & 1.03 (s, 3H), 0.87 & 0.77 (t, 3H).

1. concentrationThe reaction mixture was concentrated to dryness
2. customthe residue was partitioned between water and ethyl acetate
3. dry with materialThe organic layer was dried over Na2SO4
4. concentrationconcentrated under reduced pressure
5. customto afford 270 mg of the crude acid
6. extractionextracted with 10% MeOH in DCM
7. dry with materialThe combined organic phases were dried over Na2SO4
8. concentrationconcentrated
9. customthe residue purified by silica gel column chromatography