反应详情

温度

20 °C

To a solution of Example 56 (0.261 g) in a mixture of water (4 ml) and methanol (12 ml) was added a solution of sodium hydroxide (0.054 g) in water (0.5 ml) and the mixture was stirred at 20° C. for 72 h. The pH of the mixture was adjusted to approximately 6 by the addition of 2N hydrochloric acid and the mixture was purified by solid phase extraction (10 g SCX cartridge), eluting with methanol followed by 10% triethylamine in methanol. Evaporation of the basic fraction in vacuo gave the title compound as a colourless gum, (0.319 g).

1. customthe mixture was purified by solid phase extraction (10 g SCX cartridge)
2. washeluting with methanol
3. customEvaporation of the basic fraction in vacuo