Tromethamine hydrochloride → Prostaglandin D2

HRID 2213693Notes

Reaction equation

HRID 2213693 reaction equation
C(O)C(N)(CO)CO.Cl.[Mg+2].[Cl-].[Cl-].[CH3:13][CH2:14][CH2:15][CH2:16][CH2:17][C@H:18]([OH:37])/[CH:19]=[CH:20]/[C@H:21]1[C:25](=[O:26])[CH2:24][C@H:23]([OH:27])[C@@H:22]1[CH2:28]/[CH:29]=[CH:30]/[CH2:31][CH2:32][CH2:33][C:34]([OH:36])=[O:35]>>[CH3:13][CH2:14][CH2:15][CH2:16][CH2:17][C@H:18]([OH:37])/[CH:19]=[CH:20]/[C@H:21]1[C:25](=[O:26])[CH2:24][C@H:23]([OH:27])[C@@H:22]1[CH2:28]/[CH:29]=[CH:30]\[CH2:31][CH2:32][CH2:33][C:34]([OH:36])=[O:35]

Participants

4

Reactant 3

HCID 93573Tromethamine hydrochlorideHCID 5360315Magnesium ChlorideHCID 6519333(E)-7-[(1R,2R,5S)-5-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxocyclopentyl]hept-5-enoic acid

Product 1

HCID 448457Prostaglandin D2

Procedure

To a binding-reaction solution (50 mM Tris/HCl, pH 7.4, 5 mM MgCl2) (0.2 ml) were added the human platelet membrane fraction (0.1 mg) and 5 nM [3H]PGD2 (115 Ci/mmol), and the mixture was reacted at 4° C. for 90 min. After the reaction, the mixture was filtered through a glass fiber filter paper and washed several times with cooled physiological saline, then the radioactivity retained on the filter paper was measured. The specific-binding ratio was calculated by subtracting the non-specific binding ratio which is the radioactivity similarly measured in the presence of 10 μM PGD2 from the total binding. The inhibitory activity of each compound was expressed as the concentration required for 50% inhibition (IC50), which was determined by depicting a substitution curve by plotting the binding ratio (%) in the presence of each compound, where the binding ratio in the absence of a test compound is 100%.

Workup

  1. customthe mixture was reacted at 4° C. for 90 min
  2. customAfter the reaction
  3. filtrationthe mixture was filtered through a glass fiber
  4. filtrationfilter paper
  5. washwashed several times
  6. temperaturewith cooled physiological saline
  7. concentrationThe inhibitory activity of each compound was expressed as the concentration

Sources & evidence

ORD record
ord-1c45e1751b2f4900919518bf2c740f38
Source dataset
uspto-grants-2006_09
Patent number
US07105564B1

Reaction notes

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