L-Proline → cis-3-Hydroxy-L-proline

HRID 2216242Notes

Reaction equation

HRID 2216242 reaction equation
[NH:1]1[CH2:8][CH2:7][CH2:6][C@H:2]1[C:3]([OH:5])=[O:4].[O:9]=C(CCC([O-])=O)C([O-])=O>>[OH:9][C@@H:6]1[CH2:7][CH2:8][NH:1][C@@H:2]1[C:3]([OH:5])=[O:4]

Participants

3

Reactant 2

HCID 145742L-ProlineHCID 164533Alpha-Ketoglutarate

Product 1

HCID 7098652cis-3-Hydroxy-L-proline

Procedure

The enzyme reaction by use of purified L-proline-3-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM TES buffer (pH 7.0), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acids 1 mM, ferrous sulfate, and 106 U of purified enzyme preparation, the total volume being 100 μl. The reaction was carried out at 35° C. for 30 minutes. As a result of the reaction, 18 mM (2.4 g/l) of cis-3-hydroxy-L-proline was produced in the reaction mixture.

Workup

  1. customobtained in Example 5
  2. customAs a result of the reaction

Sources & evidence

ORD record
ord-96a4f8d45aa84662b0c6d7bd935c90b1
Source dataset
uspto-grants-2006_09
Patent number
US07112432B2

Reaction notes

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