Succinic Acid → Malic Acid

HRID 2220789Notes

Reaction equation

HRID 2220789 reaction equation
[O:1]=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.C(=O)([O-])[O-].[Na+].[Na+].[C:19]([OH:26])(=[O:25])[CH2:20][CH2:21][C:22]([OH:24])=[O:23]>>[C:19]([OH:26])(=[O:25])[CH:20]([CH2:21][C:22]([OH:24])=[O:23])[OH:1]

Participants

4

Reactant 3

HCID 1110Succinic AcidHCID 10340Sodium CarbonateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 525Malic Acid

Conditions

Temperature
33 °C

Procedure

The coryneform bacterium cells prepared after culturing were added to 500 ml of the reaction stock solution in a reaction container under the nitrogen gas atmosphere. Glucose 200 mM and sodium carbonate 200 mM were added, and the reaction temperature was maintained at 33° C. to perform a reaction producing an organic compound. An oxidation-reduction potential was initially −200 mV, but was reduced immediately after the initiation of the reaction, and the reaction was continued by maintaining the oxidation-reduction potential at −400 mV. After the reaction for 3 hours, the reaction medium solution was analyzed by liquid chromatography, indicating that succinic acid 163 mM (19.2 g/L) and malic acid 5 mM (0.67 g/L) were produced. Lactic acid was not detected.

Workup

  1. customThe coryneform bacterium cells prepared after culturing
  2. additionwere added to 500 ml of the reaction stock solution in a reaction container under the nitrogen gas atmosphere
  3. customproducing an organic compound
  4. customafter the initiation of the reaction
  5. temperatureby maintaining the oxidation-reduction potential at −400 mV
  6. customAfter the reaction for 3 hours

Sources & evidence

ORD record
ord-62aa3bd72c4b4c32b0af22b6152624ae
Source dataset
uspto-grants-2008_05
Patent number
US07368268B2

Reaction notes

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