Hydrochloric Acid → HCID 45122314, compound

HRID 2226359Notes

Reaction equation

HRID 2226359 reaction equation
[ClH:1].[C:2]([N:5]1[CH2:12]C[CH2:10][C@H:6]1[C:7]([OH:9])=[O:8])(=[O:4])[CH3:3]>>[Cl:1][CH2:3][C:2]([N:5]([CH3:12])[C@H:6]([C:7]([OH:9])=[O:8])[CH3:10])=[O:4]

Participants

3

Reactant 2

HCID 313Hydrochloric Acid2×HCID 66141N-Acetyl-L-proline

Product 1

HCID 45122314HCID 45122314, compound

Procedure

1 ml of a 30 mM solution of N-choloracetyl-N-methyl-L-alanine in 0.1M tris-HC1, pH 7.0 was introduced into a reaction container thermostatted to 30° C. and mixed with 1.99 ml 0.1M tris --HCl pH 7.0. The reaction was started by adding 0.01 ml N-acyl-L-proline acylase from Comamonas TESTOSTERONI DSM 5416 corresponding to 0.23 units and 0.2 μg protein. 1 unit is defined as the amount of enzyme which converts 1 μmole of N-acetyl-L-proline per minute at 30° C. and pH 7.0. Specimens were taken at various points in time to detect the course of the reaction, compounded with 2N HCl 1:2 and subsequently analyzed by HPLC using an RP 8-column (250×4 mm). After elution with 7% (v/v) acetonitrile in 0.01M heptane sulfonic acid (adjusted to pH 2.2 with 85% H3PO4) at a flow rate of 2.2 ml/min. and a column temperature of 40° C., the decrease of N-chloroacetyl-N-methyl-L-alanine was determined by measuring the UV absorption at 210 nm.

Workup

  1. additionto 30° C. and mixed with 1.99 ml 0.1M
  2. customat 30° C.
  3. customthe course of the reaction
  4. washAfter elution with 7% (v/v) acetonitrile in 0.01M heptane sulfonic acid (adjusted to pH 2.2 with 85% H3PO4) at a flow rate of 2.2 ml/min.
  5. customa column temperature of 40° C.
  6. customthe UV absorption at 210 nm

Sources & evidence

ORD record
ord-5fcc26a727a4489b88ef30759d6126ef
Source dataset
uspto-grants-1994_09
Patent number
US05348882

Reaction notes

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