(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → L-Leucine

HRID 2227141Notes

Reaction equation

HRID 2227141 reaction equation
O=[CH:2][C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H]([O:37][C@@H:38]2[O:43][C@@H:42](CO)[C@H:41](O)[C@@H:40](O)[C@@H:39]2[NH:48]C)[C@@]1(O)C=O>>[NH2:48][C@H:39]([C:38]([OH:37])=[O:43])[CH2:40][CH:41]([CH3:42])[CH3:2]

Participants

3

Reactant 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 178484872HCID 178484872, compound

Product 1

HCID 6106L-Leucine

Procedure

pseudomesenteroides was cultivated in 10 ml M17 (0.5% glucose) for 3 days followed by cultivation for 120 minutes in the presence of 150 μl of EMS. After EMS treatment, 0.2 ml of the culture was inoculated into ten tubes each containing 10 ml of M17 and incubated for 3 days for phenotypic expression. The mutation frequency was monitored by plating 0.1 ml from each tube onto M17 plates containing 500 μg/ml of streptomycin.

Workup

  1. waitincubated for 3 days for phenotypic expression

Sources & evidence

ORD record
ord-bf436d4d78d543a3b07da6dbed20c072
Source dataset
uspto-grants-2002_07
Patent number
US06413765B1

Reaction notes

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