HCID 178498410, compound → Nifedipine

HRID 2231811Notes

Reaction equation

HRID 2231811 reaction equation
[CH3:1][C:2]1[NH:7][C:6]([CH3:8])=[C:5]([C:9]([O:11][CH3:12])=[O:10])[CH:4]([C:13]2[CH:14]=[CH:15][CH:16]=[CH:17][C:18]=2[N+:19]([O-:21])=[O:20])[C:3]=1[C:22]([O:24][CH3:25])=[O:23].[N-]=C=S>C(=O)(O)[O-].[Na+].C(O)C>[CH3:1][C:2]1[NH:7][C:6]([CH3:8])=[C:5]([C:9]([O:11][CH3:12])=[O:10])[CH:4]([C:13]2[CH:14]=[CH:15][CH:16]=[CH:17][C:18]=2[N+:19]([O-:21])=[O:20])[C:3]=1[C:22]([O:24][CH3:25])=[O:23]

Participants

4

Reactant 1

HCID 178498410HCID 178498410, compound3×

Product 1

HCID 4485Nifedipine

Reagents, catalysts & solvents 2

HCID 702EthanolSolvent2×HCID 516892Sodium BicarbonateSolvent

Procedure

Nifedipine-isothiocyanate was also coupled to casein, ovalbumin and keyhole limpet hemocyanin using the procedures described above. An alternate procedure for the coupling of nifedipine-isothiocyanate to various proteins (i.e., bovine serum albumin, ovalbumin, casein and keyhole limpet hemocyanin) was to dissolve the protein (20 mg) in 2 ml of 100 mM sodium bicarbonate (pH 9.0) and then incubate with 0.8 mg of nifedipine-isothiocyanate (dissolved in ethanol to make the final concentration of ethanol in the incubation medium 38 percent) for 24 hours at 37° C. with shaking in the dark. This alternate procedure is useful for producing nifedipine conjugates at high protein concentrations.

Workup

  1. customfor 24 hours
  2. customat 37° C.

Sources & evidence

ORD record
ord-e3107ca6143048338fb692486198a554
Source dataset
uspto-grants-1988_11
Patent number
US04784955

Reaction notes

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