反应详情

To a suspension of 50 mg 1-[5-(5-chloro-thiophen-2-yl)-isoxazol-3-ylmethyl]-1H-pyrrolo[3,2-b]pyridine-2-carboxylic acid, 36 mg 1-isopropyl-piperidin-4-ylamine hydrochloride and 35 mg bis(2-oxo-3-oxazolidinyl)phosphinic chloride in 1 mL dichloromethane, 77 μl triethylamine were added at room temperature and the mixture was stirred for 16 hours. After removing of the solvent under reduced pressure, the residue was purified by preparative HPLC (C18 reverse phase column, elution with a water/acetonitrile gradient with 0.1% trifluoroacetic acid). The fractions containing the product were evaporated and lyophilized to yield a white residue which was partitioned between 5 mL aqueous 0.1 N sodium hydroxide solution and 5 mL ethyl acetate. The organic layer was washed with additional water and then dried over sodium sulphate. After filtration and removal of the solvent under reduced pressure, a white solid was obtained.

1. additionwere added at room temperature
2. customAfter removing of the solvent under reduced pressure
3. customthe residue was purified by preparative HPLC (
4. washC18 reverse phase column, elution with a water/acetonitrile gradient with 0.1% trifluoroacetic acid)
5. additionThe fractions containing the product
6. customwere evaporated
7. customto yield a white residue which
8. customwas partitioned between 5 mL aqueous 0.1 N sodium hydroxide solution and 5 mL ethyl acetate
9. washThe organic layer was washed with additional water
10. dry with materialdried over sodium sulphate
11. filtrationAfter filtration and removal of the solvent under reduced pressure
12. customa white solid was obtained