反应详情

2-Amino-3-((1-(3-((1-(triphenylmethyl)imidazol-2-yl)amino)propyl)(1H-indazol-5-yl))carbonylamino)propanoic acid (0.203 g, 0.331 mmol) was dissolved in trifluoroacetic acid (3 mL), and the reaction was refluxed for 1 h. The reaction was concentrated to an oil under high vacuum. The oil was triturated with ether. The product was filtered, washed with ether, dissolved in 50/50 acetonitrile/water, and lyophilized to give 171.0 mgs (106%) of product. ESMS: Calcd. for C17H21N7O3, 371.17. Found, 372.0 [M+H]+1 Analytical HPLC, Method B, Rt=9.48 min, Purity=95%.

1. temperaturethe reaction was refluxed for 1 h
2. concentrationThe reaction was concentrated to an oil under high vacuum
3. customThe oil was triturated with ether
4. filtrationThe product was filtered
5. washwashed with ether
6. dissolutiondissolved in 50/50 acetonitrile/water