Water → HCID 178518226, compound

Temperature

40 °C

To a solution of 1.0 g of 4-hydroxypyrrolo[2,3-d]pyrimidine in 20 ml of DMF, 3.8 g (2.5 eq) of bis(trimethylsilyl)acetamide was added, and the resulting solution was stirred at 40° C. in an oil bath for about two hours. Completeness of silylation was indicated by NMR analysis of an aliquot showing disappearance of the N-3 proton signal. The reaction was cooled to ambient temperature and 1.6 g (1.2 eq) of N-bromosuccinimide (NBS), was added in one portion. The reaction mixture was protected from light and stirred at ambient temperature until completion was indicated by NMR analysis (disappearance of pyrrole C-H doublets and emergence of a single, finely split doublet at δ7.16, about 2 h). The mixture was poured into 50 mL of water, with stirring. After 1-2 hours, the product was collected by filtration, washed with water, dried, and reslurried in 10 volumes of 10% methanol in chloroform. Filtration and drying gave 1.2 g of 4-hydroxy-5-bromopyrrolo-[2,3-d]pyrimidine (75% yield), mp 269-°271° C. (dec). 1H NMR (300 MHz, DMSO-d6) δ7.16 (d, J=2.0 Hz, 1 H), 7.80 (d, J=2.1 Hz, 1 H), 11.87 (s, 1 H), 12.15 (s, 1 H). 13C NMR (75.5 MHz, DMSO-d6) 89.3, 105.5, 120.4, 144.3, 147.4, 157.5.

1. temperatureThe reaction was cooled to ambient temperature
2. stirringstirred at ambient temperature until completion
3. customat δ7.16, about 2 h
4. stirringwith stirring
5. waitAfter 1-2 hours
6. filtrationthe product was collected by filtration
7. washwashed with water
8. customdried
9. filtrationFiltration
10. customdrying

ORD record

ord-aac3098560e546ef8a20401b990cd299

Source dataset

uspto-grants-1993_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05235053