Phosphate → D-(-)-Fructose

HRID 2255354Notes

Reaction equation

HRID 2255354 reaction equation
P([O-])([O-])([O-])=O.[O:6]=[CH:7][C@H:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11])[OH:9]>O>[OH:6][CH2:7][C:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11])=[O:9]

Participants

4

Reactant 2

HCID 1061PhosphateHCID 161658Demannose

Product 1

HCID 5984D-(-)-Fructose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Into 0.5 ml of a 0.1M phosphate buffer (pH 7.0) containing 0.2M mannose dissolved therein is added a suitable amount of the enzyme, the total volume is made up with water to 1.0 ml, and the reaction mixture is incubated at 50° C. The amount of fructose formed is determined by the cysteine-carbazole method. The amount of the enzyme forming 1 micromole of fructose per one minute under this condition is defined as one unit.

Workup

  1. dissolutiondissolved
  2. additiontherein is added a suitable amount of the enzyme
  3. customis incubated at 50° C

Sources & evidence

ORD record
ord-82470bbf56e24c8eafebb066f1265092
Source dataset
uspto-grants-1993_08
Patent number
US05240717

Reaction notes

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