Phosphate → D-(-)-Fructose

Participants

Reaction Steps

Into 0.5 ml of a 0.1M phosphate buffer (pH 7.0) containing 0.2M mannose dissolved therein is added a suitable amount of the enzyme, the total volume is made up with water to 1.0 ml, and the reaction mixture is incubated at 50° C. The amount of fructose formed is determined by the cysteine-carbazole method. The amount of the enzyme forming 1 micromole of fructose per one minute under this condition is defined as one unit.

Provenance & Evidence