(2S)-2-amino-3-(3-amino-4-hydroxyphenyl)propanoic acid;5-amino-2,3-dihydrophthalazine-1,4-dione → Luminol
Reaction equation
[CH:1]1[CH:6]=[C:5]([NH2:7])[C:4]2[C:8]([NH:10][NH:11][C:12](=[O:13])[C:3]=2[CH:2]=1)=[O:9].C1C(C[C@H](N)C(O)=O)=CC(N)=C(O)C=1>CS(C)=O.O>[CH:1]1[CH:6]=[C:5]([NH2:7])[C:4]2[C:8]([NH:10][NH:11][C:12](=[O:13])[C:3]=2[CH:2]=1)=[O:9]Participants
4
Reactant 1
Product 1
Reagents, catalysts & solvents 2
Procedure
Degreased glass microscope slides were coated on one side with clear epoxy cement (two-component "do it yourself", commercially available epoxy cement), which was allowed to harden. The epoxy-coated slides were then immersed in a DALM-forming solution of 15 ml of luminol in DMSO (solution A), 15 ml of 3AT in water (solution B), and 3 ml of sodium nitrite in water (solution C), for seven days. The slides were removed from the DALM-forming solution, rinsed with deionized water, then activated by placing them in a solution of 8 ml of 0.3M sodium bicarbonate solution and 8 ml of 3% hydrogen peroxide for seven days. The slides were removed from the bicarbonate/peroxide solution, rinsed with deionized water and blotted dry.
Sources & evidence
- ORD record
- ord-99c2875dbcbc4d6e8400099249dd13ab
- Source dataset
- uspto-grants-1997_08
- Patent number
- US05658673
Reaction notes
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